LPS通过AKT/FOXO1信号通路诱导C2C12肌管细胞MuRF1基因转录

doi:10.11843/j.issn.0366-6964.2016.02.022

Abstract

Abstract:

This study was designed to investigate the effects of the inflammatory induced by lipopolysaccharide (LPS) on the genes and pathways controlling the protein degradation in C2C12 myotubes.In order to establish an inflammatory cell mode，the C2C12 myotubes were treated with graded concentrations of LPS (10，100 and 1 000 ng•mL^-1) for 30 min and 3 h，and then the mRNA transcription of TNF-α and IL-6 were determined by qPCR method.The results showed that 1 000 ng•mL^-1 LPS was the optimal concentration.After the C2C12 myotubes were treated with 1 000 ng•mL^-1 LPS for 0 min，30 min，1 h，3 h，6 h，12 h and 24 h，the mRNA transcription of MAFbx and MuRF1 were determined by qPCR.And the activation of AKT/FOXO1，mTOR and p38MAPK signaling pathway was detected by western blot at 12 h after LPS treatment.The results showed that 1 000 ng•mL^-1 LPS significantly induced the mRNA transcription of TNF-α and IL-6 at 30 min and 3 h.The transcription of MuRF1，IL-1β，IL-6 and TLR4 were significantly up-regulated at 12 h and 24 h after LPS treatment.In addition，the phosphorylation of AKT and FOXO1 in C2C12 myotubes was significantly suppressed at 12 h after LPS treatment.Therefore，our results suggest that LPS can induce MuRF1 expression in C2C12 myotubes through the regulation of AKT/FOXO1 signaling pathway.

CLC Number:

S852.2

LIU Yan，LIANG Hui-huang，LIU Yu-lan，TANG Zhong-lin，WANG Wen-jun，ZHANG Jing. LPS Induces MuRF1 Transcription through AKT/FOXO1 Mediating Pathway in C2C12 Myotubes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 374-380.

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LPS Induces MuRF1 Transcription through AKT/FOXO1 Mediating Pathway in C2C12 Myotubes

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